rabbit antiphosphorylate ampkα2-t172 monoclonal antibody 2535t  (Cell Signaling Technology Inc)

Journal: ACS Omega

Article Title: Injectable Thermosensitive Hydrogel Containing Resveratrol-Laden Nanoparticles Promotes Hypertrophic Scar Repair in Rat Tail

doi: 10.1021/acsomega.5c01469

Figure Lengend Snippet: (A) Representative immunofluorescence-staining images of phosphorylated p38-MAPK expression in scar tissue at week 0, week 2, and week 6 post-treatments. Scale bar = 200 or 50 μm. (B) ROS activity in scar tissue at week 0, week 2, and week 6 post-treatments. (C) Quantitative evaluation of phosphorylated p38-MAPK expression in scar tissue. Notes: compared with normal skin (NS), ** P < 0.01, *** P < 0.001,**** P < 0.0001; compared with NC, △△ P < 0.01, △△△△ P < 0.0001; compared with MSN@Res, #### P < 0.0001.

Article Snippet: Subsequently, they were kept at 4 °C overnight in the presence of rabbit antiphosphorylated p38-MAPK (p-p38, bs-5476R, Bioss, China, 1:400), rabbit anti-LC3-II (LC3-II, 14600–1-AP, Aspen, China, 1:500), rabbit anti-cleaved caspase-3 (Cleaved Caspase 3, 25128–1-AP, Aspen, China, 1:150), rodent-derived p53 antibody (p53, 60283–2-IG, Aspen, China, 1:400), and a rat-originated HIF-1α antibody (HIF-1α, SC-13515, Santa Cruz, 1:100).

Techniques: Immunofluorescence, Staining, Expressing, Activity Assay